Avian infectious bronchitis virus ( IBV ) is a member of the group III coronaviruses ,
the recombinant protein was cloned ,
Avian infectious bronchitis virus ( IBV ) is a member of the group III coronaviruses , which differ from the other groups of coronaviruses in that they do not encode the essential pathogenic factor nonstructural protein 1 ( nsp1 ) and instead start with nsp2 .
In order to better understand the structural details and functional mechanism of IBV nsp2 , the recombinant protein was cloned , overexpressed in Escherichia coli , purified and crystallized .