The analytical sensitivity of the RespoCheck triplex real - time PCR was , <*> 300 colony forming units ( cfu)/mL for M. dispar , and 30 cfu / mL for M. bovis or M. bovirhinis .
The analytical sensitivity of the RespoCheck Mycoplasma triplex real - time PCR was , <*> 10 fg DNA per assay for M. dispar and 100 fg fo rM. bovis and M. bovirhinis .
The analytical sensitivity of the RespoCheck triplex real - time PCR was , as determined by spiking experiments of the Mycoplasma strains in Phosphate Buffered Saline , 300 colony forming units ( cfu)/mL for M. dispar , and 30 cfu / mL for M. bovis or M. bovirhinis .
The analytical sensitivity of the RespoCheck Mycoplasma triplex real - time PCR was , as determined on purified DNA , 10 fg DNA per assay for M. dispar and 100 fg fo rM. bovis and M. bovirhinis .