The approach resulted in the identification of two hits , LASSBio-1799 ( 7 ) and LASSBio-1814 ( 10 ) ,
This study also enabled the determination of the structural requirements for interactions with the active site of PI4KIIIβ ,
The approach resulted in the identification of two hits , LASSBio-1799 ( 7 ) and LASSBio-1814 ( 10 ) , which inhibited the target enzyme with IC 50 values of 3.66 μM and IC 50 and 6.09 μM , respectively .
This study also enabled the determination of the structural requirements for interactions with the active site of PI4KIIIβ , demonstrating the importance of both acceptor and donor hydrogen bonding groups for forming interactions with binding site residues Val598 and Lys549 .