Additional evidence for the pivotal role of extracellular cysteines in the APN molecule was obtained
Enzymatically inactive cysteine mutants were retained in the endoplasmic reticulum
Additional evidence for the pivotal role of extracellular cysteines in the APN molecule was obtained when substitution of any of these six cysteines caused complete loss of surface expression and enzymatic activity .
Enzymatically inactive cysteine mutants were retained in the endoplasmic reticulum as shown by high - resolution imaging and Endoglycosidase H digestion .