In this study , we aimed to generate marker - free pbd-2 knock - in pigs
Cloned piglets were produced via somatic cell nuclear transfer
In this study , we aimed to generate marker - free pbd-2 knock - in pigs using the CRISPR / Cas9 and Cre / loxP systems .
Cloned piglets were produced via somatic cell nuclear transfer and correct insertion of pbd-2 genes was confirmed by PCR and Southern blot .