Chemical denaturant sensitivity of the dimeric main protease from severe acute respiratory syndrome ( SARS ) coronavirus to guanidinium chloride was examined in terms of fluorescence spectroscopy , circular dichroism , analytical ultracentrifuge , and enzyme activity change .
We provide direct evidence for the functional role of C - terminal domain in stabilization of the catalytic N - terminal domain of SARS coronavirus main protease .
Chemical denaturant sensitivity of the dimeric main protease from severe acute respiratory syndrome ( SARS ) coronavirus to guanidinium chloride was examined in terms of fluorescence spectroscopy , circular dichroism , analytical ultracentrifuge , and enzyme activity change .
We provide direct evidence for the functional role of C - terminal domain in stabilization of the catalytic N - terminal domain of SARS coronavirus main protease .