These methods are now widely replaced with appropriate DNA - and RNA - based amplification techniques , in particular real time PCR amplification , for the detection of an extended number of agents responsible for acute respiratory infections .
the laboratory can optimise its diagnostic strategy
These methods are now widely replaced with appropriate DNA - and RNA - based amplification techniques , in particular real time PCR amplification , for the detection of an extended number of agents responsible for acute respiratory infections .
In applying molecular methods to diagnostic use , the laboratory can optimise its diagnostic strategy by applying a combination of real - time amplification tests for respiratory viruses and the non - viral respiratory bacterial pathogens .