The analytical sensitivity of the RespoCheck triplex real - time PCR was , <*> 300 colony forming units ( cfu)/mL for M. dispar , and 30 cfu / mL for M. bovis or M. bovirhinis .
The analytical specificity of the RespoCheck Mycoplasma triplex real - time PCR was , <*> 100 , 98.2 and 99.1 % for M. bovis , M. dispar and M. bovirhinis respectively .
The analytical sensitivity of the RespoCheck triplex real - time PCR was , as determined by spiking experiments of the Mycoplasma strains in Phosphate Buffered Saline , 300 colony forming units ( cfu)/mL for M. dispar , and 30 cfu / mL for M. bovis or M. bovirhinis .
The analytical specificity of the RespoCheck Mycoplasma triplex real - time PCR was , as determined by testing Mycoplasmas strains ( n = 17 ) and other bacterial strains ( n = 107 ) , 100 , 98.2 and 99.1 % for M. bovis , M. dispar and M. bovirhinis respectively .