In this study we evaluated the RespoCheck Mycoplasma triplex real - time PCR for the detection in bronchoalveolar lavage fluid ( BALF ) of Mycoplasma ( M. ) dispar , M. bovis and M. bovirhinis ,
The analytical sensitivity of the RespoCheck triplex real - time PCR was , <*> 300 colony forming units ( cfu)/mL for M. dispar , and 30 cfu / mL for M. bovis or M. bovirhinis .
In this study we evaluated the RespoCheck Mycoplasma triplex real - time PCR for the detection in bronchoalveolar lavage fluid ( BALF ) of Mycoplasma ( M. ) dispar , M. bovis and M. bovirhinis , all three associated with bovine respiratory disease ( BRD ) .
The analytical sensitivity of the RespoCheck triplex real - time PCR was , as determined by spiking experiments of the Mycoplasma strains in Phosphate Buffered Saline , 300 colony forming units ( cfu)/mL for M. dispar , and 30 cfu / mL for M. bovis or M. bovirhinis .