Two copies of pbd-2 gene <*> were inserted into the porcine Rosa26 locus through CRISPR / Cas9-mediated homology - directed repair .
The floxed selectable marker gene neoR , <*> was removed by cell - penetrating Cre recombinase with a recombination efficiency of 48.3 % .
Two copies of pbd-2 gene linked by a T2A sequence were inserted into the porcine Rosa26 locus through CRISPR / Cas9-mediated homology - directed repair .
The floxed selectable marker gene neoR , used for G418 screening of positive cell clones , was removed by cell - penetrating Cre recombinase with a recombination efficiency of 48.3 % .