Chemical denaturant sensitivity of the dimeric main protease from severe acute respiratory syndrome ( SARS ) coronavirus to guanidinium chloride was examined in terms of fluorescence spectroscopy , circular dichroism , analytical ultracentrifuge , and enzyme activity change .
The dimeric enzyme dissociated at guanidinium chloride concentration of , 0.4 M ,
Chemical denaturant sensitivity of the dimeric main protease from severe acute respiratory syndrome ( SARS ) coronavirus to guanidinium chloride was examined in terms of fluorescence spectroscopy , circular dichroism , analytical ultracentrifuge , and enzyme activity change .
The dimeric enzyme dissociated at guanidinium chloride concentration of , 0.4 M , at which the enzymatic activity loss showed close correlation with the subunit dissociation .