This potency was 65-fold higher than the potency of the lead compound 4 ( K i ¼ 0.39 mM ) .
In addition , the K i value of 5h was in very good agreement with the binding affinity ( 16 nM )
This potency was 65-fold higher than the potency of the lead compound 4 ( K i ¼ 0.39 mM ) .
In addition , the K i value of 5h was in very good agreement with the binding affinity ( 16 nM ) observed in isothermal titration calorimetry ( ITC ) .