Chemical denaturant sensitivity of the dimeric main protease from severe acute respiratory syndrome ( SARS ) coronavirus to guanidinium chloride was examined in terms of fluorescence spectroscopy , circular dichroism , analytical ultracentrifuge , and enzyme activity change .
Different mutants of the full - length protease <*> with tryptophanyl residue(s )
Chemical denaturant sensitivity of the dimeric main protease from severe acute respiratory syndrome ( SARS ) coronavirus to guanidinium chloride was examined in terms of fluorescence spectroscopy , circular dichroism , analytical ultracentrifuge , and enzyme activity change .
Different mutants of the full - length protease ( W31 and W207 / W218 ) , with tryptophanyl residue(s ) mutated to phenylalanine at the C - terminal or N - terminal domain , respectively , were constructed .