Unfolding curves of these mutants were monophasic
The unfolding intermediate of the protease thus represented a folded C - terminal domain but an unfolded N - terminal domain ,
Unfolding curves of these mutants were monophasic but corresponded to the first and second phases of the protease , respectively .
The unfolding intermediate of the protease thus represented a folded C - terminal domain but an unfolded N - terminal domain , which is enzymatically inactive due to loss of regulatory properties .