In this study , we aimed to generate marker - free pbd-2 knock - in pigs
Two copies of pbd-2 gene <*> were inserted into the porcine Rosa26 locus through CRISPR / Cas9-mediated homology - directed repair .
In this study , we aimed to generate marker - free pbd-2 knock - in pigs using the CRISPR / Cas9 and Cre / loxP systems .
Two copies of pbd-2 gene linked by a T2A sequence were inserted into the porcine Rosa26 locus through CRISPR / Cas9-mediated homology - directed repair .