Field studies on inhibition of Haemonchus contortus. The epidemiology of Haemonchus contortus was studied in north east England in lambs reared under field conditions with infected ewes. The parasite population was monitored by pasture larval counts, faecal egg counts and lamb postmortems. From 25 June to 11 November, eight lambs were slaughtered each month, together with eight worm-free tracer lambs. Postmortem worm counts showed that on 25 June, small numbers of adult H. contortus were present but no inhibited larvae. However, subsequently the number of worms recovered rose rapidly to 1000-5000 and the percentage inhibition increased to 57% in July, 75% in August and virtually 100% in September, October and November. On each sampling occasion no significant differences in worm count or percentage inhibition were recorded between experimental and tracer lambs. These results suggest that neither autumn climatic effects nor host immunity were responsible for inhibition in this strain of H. contortus.


Histochemical demonstration of acetylcholinesterase in sporocysts of Schistosoma mansoni (Trematoda). The distribution of acetylcholinesterase in mother and daughter sporocysts of Schistosoma mansoni was studied histochemically. In young mother sporocysts derived from miracidia cultured in vitro the miracidial neural mass and flame cells were shown to persist. The nerve trunks and commissures, as well as papillae, are apparently lost in the transformation process. In young daughter sporocysts freshly dissected from mother sporocysts there was little enzyme activity except for a sparse distribution in the tegument. After cultivation, intense enzyme activity was associated with developing cercarial embryos. A similar distribution of activity was observed in older daughter sporocysts obtained from the digestive gland of snails. No evidence of flame cells, neural mass, or commissures was detected in daughter sporocysts by the methods employed.


Utilization of amino acids by Trypanosoma brucei in culture: L-threonine as a precursor for acetate. The amino acid compositions of several culture media have been analysed and compared. The utilization and excretion of amino acids and other metabolites have been followed during growth of Trypanosoma brucei S42 in a defined medium. All of the added L-threonine was metabolized by the cells, even when it was present at elevated concentrations. Glucose was consumed throughout the growth cycle: glutamine was consumed more rapidly than glutamic acid, which was itself used at about the same rate as proline. Threonine was cleaved to form glycine and acetate, both of which accumulated in the medium. Alanine and succinate were excreted together with a small amount of pyruvate, but these three products accounted for less than half of the glucose used. CO2 production from glucose was not measured, but insignificant amounts of CO2 were produced from threonine. Tetraethylthiuram disulphide blocked the cleavage of threonine and was a potent inhibitor of trypanosome growth.


Schistosoma mansoni: in vitro conversion of cercariae to schistosomula. Schistosoma mansoni cercariae were incubated for 3-5h at 37 degrees C in various test solutions, and the rate and extent of conversion of the cercariae to schistosomula were determined. Criteria used to identify schistosomula included: (1) loss of cercarial tail, (2) viability of organisms in saline but not in water, (3) pre-acetabular gland evacuation and (4) ability to survive in culture. Incubation of cercariae in rat chamber fluid resulted in organisms which were water sensitive, but retained their tails and pre-acetabular gland contents. Conversion to water sensitivity was not blocked by 0-01 M EDTA.


Effect of temporary hypoxia on the permeability of the rat aorta. Hypoxia was produced experimentally in the infrarenal portion of rat abdominal aorta by ligating it at both ends for one hour. Permeability changes of the injured segment were followed up by means of a colloidal iron tracer after different periods of recirculation. The tracer could be demonstrated in intercellular junctions and subendothelial space already after one hour of recirculation. The permeability disorder was at its peak two days after the injury and it ceased by the tenth day. Insudation of the media by plasma increased proportionally with the endothelial permeability change and it was accompanied by marked ultrastructural alterations.


Acid esterase in the aorta of the hyperlipidemic rat: a histochemical study. In rats maintained for about three weeks on a diet inducing hyperlipidemia E-600 resistant acid esterase activities were markedly reduced in the aorta in comparison with untreated animals. In rats which were maintained on an ordinary diet containing the same amount of thiouracil as given to the hyperlipidemic animals, only a slight reduction of acid esterase activities was noted. Another group of animals was fed an ordinary diet for three weeks after three weeks on a hyperlipidemic diet. In the aorta of these animals the acid esterase activities were almost normal. There was little fat deposition in the aortas of animals given the various diets. No such effects on esterase activities were observed in the liver and lung of animals of the various groups. Inhibition of acid esterase activity was also observed in rats kept on a hyperlipidemic diet for 65 days. In these animals patchy deposition of partly anisotropic lipid was observed in the intima and media.


The parathyroid glands of the Mongolian gerbil (Meriones unguiculatus). The parathyroid glands of adult Mongolian gerbils were studied under an operation microscope, as well as with the aid of conventional light microscopy, and transmission and scanning electron microscopy. The mean serum level of calcium was 5.1 mEq/1 and that of phosphorus was 4.8 mg/100 ml. The animals possessed two small parathyroid glands, usually situated anteriorly and slightly laterally to the superior thyroid poles. The glands had a rich vascular supply and were composed of sheets of chief cells, which varied in electron density and content of organelles. No water-clear, oxyphil, or intraglandular fat cells were found. Some mitochondria exhibited electron lucent areas, and separation and bulbous projections of the membranes. The cell membranes were straight or tortuous and possessed microvilli, occasionally with central filamentous structures. The intercellular spaces were usually narrow. It is supposed that the variants of chief cells are interchangeable and that the differences in their morphological appearance are related to differences in functional state.


Subcellular morphological changes in the rat kidney after phosphorus burn. Experimental phosphorus burns were performed on male rats, in order to evaluate the subcellular changes which had occurred as a result of their lesions. In addition to the external wound caused by the burn itself, pathological changes were observed macroscopically and microscopically in various body organs, mainly the kidneys. These were investigated under the electron microscope for subcellular alterations at their damaged sites, and for biochemical aberrations that were observed in those rats. In the phosphorus-burnt rats the glomeruli were ischemic, showed capillary collapse and exhibited proliferation of mesangial areas and basement membrane thickening. Many necrotic cells were observed in the proximal tubule, where large vacuoles containing myelin-like structures were identified. The lumen of the proximal tubules were completely occluded by cell debris and the cytoplasm was necrotic. Due to the damage caused to the glomeruli, high concentrations of serum urea, serum SGPT and PO-4 were assayed in the phosphorus-burnt rats. These changes may account for the high mortality rate after phosphorus burns and may further understanding of the damage as well as ways of approaching it.


The effect of hypotensive drugs (Serpasil) on the histochemical reactions in the small intestine of white rats. The influence of Serpasil (Ciba) on histological activities of dehydrogenases and reductases as well as on PAS-positive substances in the small intestine of white rats has been studied. It has been demonstrated that a decrease in the succinic, lactic and glucose-6-phosphate dehydrogenase activities as well as in the NADH and NADPH tetrazole reductase activities occurs in the rat's small intestine epithelial cells after the administration of Serpasil. However, a considerable intensification in the reactions to PAS-positive substances in the mucous cells and an increase in the amount of these cells has been observed.


Phaeochromocytoma, Electron microscopic study on catecholamine storage. The biochemical and electron-microscopical findings of eight cases of phaeochromocytoma and the ultrastructure of two cases of human adrenal medulla are presented here as regards catecholamine storage. Due to the rarity of phaeochromocytoma, other ultrastructural features are also described. The morphological characteristics of catecholamine-containing granules are considered on the basis of our investigations and results of other authors. These characteristics are discussed from the point of view of their value in the morphological differentiating of granules storing norepinephrine and those storing epinephrine.


The influence of cycloheximide and chloramphenicol on rat serum proteins. The effects of cycloheximide (CHM) and chloramphenicol (CAP) on rat serum proteins and on their electrophoretic patterns have been investigated. It has been shown that total serum protein is decreased by both treatments. CHM causes a fall in transferrin and alpha2(3-8) globulin content, while CAP reduces only alpha2(3-8) globulins. Different microscopic features of hepatic steatosis are described with both drugs. The results are briefly discussed.


Alteration of metastases distribution by vaso-dilating drugs. The effects of the two vasodilating substances, dihydralazine and the adrenergic alpha-receptor blocker phenoxybenzamine, were studied on the distribution and growth of MCG-L-SS metastases from intravenously injected tumour cells. Dihydralazine increased the total number of metastases in the body and both drugs increased the number in the lungs and the liver. Both drugs also increased the total metastasis volume in the liver. The effects might be secondary to the haemodynamic effects of the drugs, e.g. a changed distribution of the blood flow, more open capillaries and/or a changed hydrostatic capillary pressure.


Effects of the number of cell divisions and of added isologous nucleic acids on ageing of normal human fibroblasts in vitro. It is a matter of debate if the life span of a normal cell strain in vitro depends on the number of cell divisions passed or simply on metabolic time in culture. We have cultured sub-strains of the same normal human foetal fibroblast strain, separated but in a common dish with a common medium. One sub-strain was forced by infrequent transfers to grow slowly, the other was transferred frequently and grew freely. At intervals, cell kinetics of the strains was registered by time-lapse photography. The results indicate that ageing in vitro, manifested by changes of cell kinetics, and the total life span in vitro for normal human fibroblasts are dependent on the number of passed cell divisions, supporting the idea of a progressive decay of the transcription mechanism. A trial to postpone ageing of normal human foetal fibroblasts in vitro was made by adding nucleic acids, extracted from other organs of the same ovum, to the medium for 7 to 14 transfers. Four to 18 transfers after the end of the treatment, the cells still revealed a kinetic pattern typical of much younger normal strains, pointing to an influence on the genes. The effect is probably due to the DNA of the nucleic acid extract.


Demecolcin and vinblastine sulphate as stathmokinetic agents for different tissues of the hairless mouse. The dose/response relationship of the kinetostatic effect of demecolcin (ColcemidR) and vinblastine sulphate (VelbeR) was estimated in the epidermis, the forestomach, the jejunum and the colon of the hairless mouse (hr/hr) at 3 hours after intraperitoneal (i.p.) injection. A dose of 0.15 mg Colcemid per animal was found to be the lowest effective dose in all the organs tested. The value did not differ much from the corresponding effective dose of Velbe. This dose of Colcemid was then used to study the accumulation of arrested mitoses during the first 4 hours after i.p. injection. An almost linear increase in the mitotic count was found up to 4 hours in the epidermis, 3.5 hours in the forestomach and 3 hours in the jejunum and the colon. A dose of 0.15 mg Colcemid per animal i.p. with an observation time of 3 hours is thus a reliable method for estimation of the mitotic rate in many organs. It is also concluded that both Colcemid and Velbe can be used as reliable stathmokinetic drugs for both epidermis, forestomach and jejunum and colon mucosa provided that correct doses and short enough accumulation time (3 hours) is used.


Diagnosis of type A Niemann-Pick's disease by conjunctival biopsy. Electron microscopic examination of the conjunctive of four children affected with Niemann-Pick's disease revealed a widespread storage process. The epithelium, the connective tissue cells, the endothelial cells and the pericytes of the capillaries are filled with lysosomal inclusions with pleomorphic structure. The nerve fibres show destructive process of the myelin sheaths and accumulation of lipid material in the Schwann cells. The extension of the lesions is similar, as shown by morphologic and morphometric analysis in early and terminal stages of the diseases. Conjunctival biopsy allows an early and secure diagnosis of Niemann-Pick's disease, and should be performed when the clinical symptoms suggest a lysosomal disorder.


Hepatic vein thrombosis in Buffalo strain female rats ingesting dimethylnitrosamine. Buffalo strain male and female rats 12 weeks of age ingested 0.0114% dimethylnitrosamine in a semisynthetic diet for 12 weeks. Hepatic vein thrombosis developed along with focal fibrosis of the liver in female rats. Male rats had focal hepatic fibrosis, but not thrombosis of hepatic veins. A high incidence of hepatic vein thrombosis has been observed previously in Buffalo strain rats given carbon tetrachloride and methylcholanthrene simultaneously.


Histochemical studies of human breast tumors: Activity of alkaline phosphatase, acid phosphatase and glucose-6-phosphate dehydrogenase. Histochemical studies of human breast tumors were performed with particular emphasis on the activity of alkaline phosphatase (AIP), acid phosphatase (AcP) and glucose-6-phosphate dehydrogenase (G6PDH). Enzyme activities in benign and malignant lesions were compared. AIP was prominent in normal mammary epithelium, limited to the myoepithelial layer in benign tumors and was absent in cords of malignant cells. AcP activity was faintly detected in normal mammary epithelium, increased in canalicular epithelium of fibroadenomas and was marked in malignant cells. G6PDH exhibited marked activity in neoplastic epithelium and the stroma of nearly all carcinomas studied, whereas in benign tumors, G6PDH activity was strictly limited to the connective tissue. The study suggests a strong correlation between G6PDH activity and malignancy. The different results obtained by various workers in this field are critically reviewed, and discussed in the light of the results of the present study.


Fine structural changes in embryonic chick heart ventricle induced by lead poisoning. The embryonic chick heart ventricle of day 11 was studied electron microscopically to learn the structural changes that develop in lead poisoning. The chick embryos were administered with 0.015 mg/egg of lead acetate at day 2. The most pronounced changes observed in the ventricle were: malformed mitochondria, disorganized, short and scanty myofibrils and abundance of swollen vacuoles. The ultrastructure of the ventricle from the control chick embryos was normal. The most frequent change noted in the ventricular tissue was an alteration in the myofibrils. This study indicates that electron microscopic changes can be induced in the embryonic chick heart ventricle by lead poisoning.


Fructose-induced hyperuricemia: observations in normal children and in patients with hereditary fructose intolerance and galactosemia. After the infusion of fructose, 0.25 g/kg body wt, the mean peak plasma uric acid level was 5.4 +/- 0.7 (SEM) mg/100 ml in six normal children and was not significantly increased compared with that of the mean basal value of 4.1 +/- 0.5 mg/100 ml. The mean blood inorganic phosphate (Pi) levels were significantly less than the mean fasting value after fructose. Blood glucose, lactic acid, and fructose levels were significantly increased after fructose, but serum magnesium levels did not change. In two patients with hereditary fructose intolerance (HFI) the peak blood uric acid levels were 12.1 and 7.6 mg/100 ml, respectively, after fructose. In both patients the blood glucose concentrations decreased 69 and 26 mg/100 ml below the fasting levels after fructose. The serum Pi level decreased 2.3 and 1.2 mg/100 ml below fasting values, decrements greater than the mean decrement in serum Pi of 0.8 +/- 0.2 mg/100 ml which occurred in six normal children. The mean uric acid excretion, expressed as milligrams per mg urinary creatinine, was 0.6 +/- 0.1 (SEM) before fructose in the normal children and increased significantly to 1.0 +/- mg/mg creatinine after fructose. In two patients with HFI the uric acid excretion increased four- to fivefold after fructose administration; the increased uric acid excretion in HFI exceeded that of normal children. In three patients with galactosemia, increases in blood uric acid levels after galactose ingestion were similar to those in normal children after fructose, but less than those in patients with HFI after fructose. The serum Pi levels decreased less in galactosemic patients after galactose administration than in patients with HFI after fructose infusion. These studies support the hypothesis that fructose-induced hyperuricemia results from degradation of adenosine monophosphate. This effect appears to be specific for fructose. The lack of hyperruricemia in galactosemia patients after galactose ingestion may be explained by the observation that galactose is phosphorylated more slowly than fructose.


The vitamin B12-deficient rat as a possible model of ketotic hyperglycinemia. The rate of oxidation to respiratory CO2 of both carbon 1 of propionate and carbon 1 of glycine was decreased significantly in vitamin B12-deficient rats, to 50% and 82% of the control rate, respectively. The activity of the glycine synthase system was reduced during vitamin B12 deficiency to 25% of control activity. Serine hydroxymethyltransferase activity was similar for vitamin B12-deficient and control rats. Plasma glycine concentration in vitamin B12-deficient rats (253 +/- 16 nmol/ml) did not differ significantly from that of control rats (226 +/- 12 nmol/ml). Propionate oxidation was significantly impaired in biotin-deficient rats. However, this impairment, to 66% of the control rate, was not as large as that generated by vitamin B12 deficiency. In contrast to the result obtained in vitamin B12-deficient animals, no significant decrease in glycine oxidation could be demonstrated in biotin-deficient animals. Plasma glycine concentration of fasted biotin-deficient rats (339 +/- 26 nmol/ml) did not differ significantly from that of their controls (371 +/- 32 nmol/ml).


Uptake, activation, and esterification of fatty acids in the small intestine of suckling rat. The small intestinal mucosal phase of fatty acid absorption was studied in suckling and adult rats. Fatty acid binding protein (FABP) is present in the cytosol of jejunal mucosa of 6-day-old rats in amounts equivalent to that found in mucosal cytosol of adult rats (16.4% and 15.0%, respectively). The percentage of oleic acid binding to FABP is the same in 6-day-old and adult rats (13.9% and 10.2%, respectively). The specific activity of jejunal microsomal oleoyl-CoA synthetase is high in the fetus, falls abruptly after birth, but increased by the third day of life to remain constant thereafter into adult life. In contrast the specific activity of acyl-CoA:monoglyceride acyltransferase is low in the fetal jejunum, gradually increases, and is significantly higher in the 6- and 12-day-old rat than in the adult. Uptake of oleic acid by jejunal slices of 6- and 11-day-old animals is three- to fivefold higher than uptake by jejunal slices prepared from adult rats. The rate of esterification of oleic acid is higher in jejunal slices from 6- and 11-day-old rats, reflecting the enhanced uptake of oleic acid.


A comparison of the effects of vicariously instigated classical conditioning and direct classical conditioning procedures. Two groups of Ss received either two or 16 paired classical conditioning trails beyond the peak CR. A third group received the same stimuli as the 16 postpeak condition but in an unpaired and random order. The stimuli in all three groups were delivered directly to S. Subsequently, all three groups, including a fourth which was not given any prior direct classical conditioning, were exposed to vicariously instigated classical conditioning. This consisted of having S observe someone (model) employed by E who received the same CS as was delivered during direct conditioning. The CS was paired with the feigned arm movement of the model, simulating a reaction to shock. This vicarious classical conditioning procedure when compared to direct classical conditioning resulted in smaller GSR magnitudes for both the CRs and UCRs. Previous experience with direct classical conditional seems to have had an attenuating effect on GSR magnitude during the vicarious situation. A postexperimental questionnaire tended to support the results, and the relationship between the present study and current classical conditioning theory is discussed.


An analysis of a rebreathing method for measuring lung volume in the premature infant. Functional residual capacity (FRC) and thoracic gas volume (TGV) were measured in 20 infants, of whom 11 were healthy preterm infants, 5 were recovering from the respiratory distress syndrome (RDS), and 4 had other pulmonary problems. In addition, some of the theoretical aspects of rebreathing techniques, including the lung to bag N2 difference at equilibrium, were studied by constructing a simple digital computer model of the system. In both the normal preterm infants and the post-RDS group, the TGV was significantly greater than the FRC (0.02 greater than P greater than 0.01), indicating the presence of trapped gas (Fig. 2). The mean time taken to reach equilibrium during rebreathng was 44 sec in the normal infants and 52 sec in the post-RDS group (Table 2). In one infant with generalized cystic lung disease, equilibrium was not achieved even after 3 min of rebreathing (Fig. 1). The computer-generated analysis of rebreathing for a normal 3-kg infant is shown in Figure 3, with an in vivo curve for comparison. The rapid equilibration of N2 was completed within 55 sec but, as in the in vivo experiments, there was a change in slope of the line afer 1.7 min. It can be seen from Table 3 that, if a small initial bag volume (11 ml) is used, 30 sec of rebreathing is adequate for equilibration only when FRC and dead space are normal, and errors would occur if either were enlarged. The computer study showed that the gradient for N2 between bag and lung at equilibrium is of the order of 0.2-0.37% which would cause a negligible error when calculating FRC and assuming that lung N2 is equal to the measured bag N2. The combined in vivo and computer studies served to validate the rebreathing technique as a method for measuring FRC, and have enabled it to be modified for use even in small or very sick infants with poor ventilation. The most useful procedure to employ would seem to be to use an initial bag volume of 150-200 ml and to continue the rebreathing for 1.5-2 min, with samples being taken for analysis at approximately 20-sec intervals.


Operant GSR conditioning with cool air reinforcement. Two groups of 20 Ss each run individually in a soundproff chamber heated to 115 F and humidified to 100%. Ss in the experimental group received brief presentation of cool air contingent upon wmitted GSRs during 20 min of acquisition. Control Ss were matched on at a time in operant rate and were yoked one at a time to receive the cool air on a response-independent schedule. Both groups increased significantly in rate of unelicited GSRs during acquisition, with the experimental Ss' curve rising more rapidly and both groups attaining equivalent terminal levels. During extinction, the groups maintained high levels of responding and did not differ. Examination of basal skin conductance data ruled our an activation hypothesis. In the control Ss, terminal response frequency was significantly correlated with percentage of fortuitous response-contigent reinforcement. It was concluded that both croups were conditioned, with the controls receiving intermittent reinforcement  sufficiently often to elevate their responding. The cool air was judged to be an effective reinforcer of unelicited GSRs, perhaps more effective than those used in previous studies.


Incorporation of (3H)thymidine into brain DNA after cerebellar damage. Changes of incorporation of [3H] thymidine into brain DNA were studied in C57BL/6J mice after perinatal neocerebellar lesion. The destruction of part of the left neocerebellar cortex caused temporary increase of the specific radioactivity of DNA extracted from neocerebellum (15.22 +/- 0.57 cpm/mg DNA vs. 4.83 +/- 0.40 cpm/mg DNA in controls), from left hemisphere (9.86 +/- 0.45 cpm/mg DNA in operated vs. 4.22 +/- 0.40 cpm/mg DNA in controls), and from right hemisphere (11.75 +/- 0.52 cpm/mg DNA in operated vs. 4.78 +/- 0.39 cpm/mg DNA in controls). The labeled DNA was localized both in glia and in neurons in different brain areas. In animals operated upon in adult age, no changes in labeling of brain DNA were observed.


Aspects of biochemical differentiation in the central nervous system. A demonstration of cell-specific patterns of development in the immature CNS is provided by examples of characteristic, cell-specific time-courses of enzyme development in different classes of brain cells isolated in highly purified form by bulk-separation from the cerebral and cerebellar cortex of the growing rat. The enzymatic analysis was carried out at the level of the nerve and glial cell lysosomes and mitochondria, two subcellular organelles crucial to the economy of all cells. The findings reveal rather similar developmental patterns for the lysosomal hydrolase N-acetyl-beta-D-glucosaminidase in neurons and glial cells of the cerebral cortex as well as in two different cerebellar nerve cell types, the Purkinje and the granule cell. However, significant differences in the post-natal chronology of development of the mitochondrial enzyme alpha-glycerophosphate dehydrogenase were noted between cortical nerve and glial cells, the glial enzyme exhibiting 6-fold higher levels of activity than the neuronal one throughout the first month of postnatal life. The findings emphasize the feasibility as well as the necessity of studies aimed at the elucidation of the cell-specific aspects of the biochemistry of developing nerve and glial cells.


Do brine shrimp diagnose cystic fibrosis? The nauplii of the brine shrimp Artemia salina are dependent upon the function of their salt gland to maintain osmotic pressure within narrow limits. A number of drugs interfere with this function and are lethal to the nauplii. Saliva and serum from normal persons, patients with cystic fibrosis, and obligate heterozygotes were tested for lethal effect against brine shrimp nauplii. At salt concentrations between 100 mM and 2.5 no difference was found among the phenotypes. At lower concentrations a difference was noted occasionally between some normal subjects and some individuals carrying one or two genes for cystic fibrosis. Data from an independent series of experiments indicate that the naupliar deaths result from distorted ratios of Na+/K+ and not from a specific gene product. No difference was noted in the O2 uptake of nauplii treated with saliva or serum obtained from normal subjects, patients with cystic fibrosis, or obligate heterozygotes.


Glucose production in the newborn dog. I. Effects of glucagon in vivo. Systemic glucose production rates were evaluated 4 hr after feeding in 14 newborn beagle dogs at ages between 1 and 5 days. After a prime injection of radioisotopic tracers, glucose production was determined during infusion of intermixed tracer [2-3H] glucose and [1-14C] glucose at a constant rate. Seven of the new born dogs served as controls throughout the 3-hr period of infusion, while seven of their littermates, infused simultaneously, received glucagon at a constant rate of 3.3 mug/min between 90 and 180 min of study. In control dogs, mean glucose production, determined by dilution of [2-3H] glucose, was 55 +/- 3 mumol/min-kg body weight. During the control period, their littermates produced glucose at a similar rate; however, glucagon infusion raised glucose production to 81 +/- 4 mumol/min-kg. The average glucose production rate estimated with [1-14C]glucose was 88% of that with 3H tracer during the initial control period and 77% during the glucagon injusion. In order to confirm that this discrepancy reflected the recycling of 14C and the early development of gluconeogenesis, and additional 14 new born dogs were infused with potential substrates for [14C] glucose. In separate studies, [U-14C] lactate, [3-14C] lactate, [U-14C] alanine, and [6-14C]- glucose were incorporated into glucose and [1-14C] glucose. Quantification of gluconeogenesis by simultaneous infusion of [6-3H] glucose and [3-14C] lactate in a 5-day-old dog demonstrated that 25% of the glucose produced originated from lactate, whereas 10% was incorporated into carbon 1. Thus, systemic glucose production was established rapidly in newborn dogs and responded to stimulation with glucagon. A significant proportion of the glucose originated from recycling via the gluconeogenic pathway.


A longitudinal study of total body potassium in normal children. Most investigators studying changes in body composition during growth agree to the desirability of having longitudinal data. A group of 110 boys and 126 girls, studied annually for 4-5 years with 880 measurements of total body K in a 2pi liquid scintillation whole body counter, had total body K vs. height and total body K vs. weight data that fit previously described regression lines.


Thyroid hormone metabolism in amniotic and allantoic fluids of the sheep. Amniotic and allantoic fluid samples were obtained at the time of uterotomy from healthy pregnant ewes at various time during gestation. In most instances maternal and fetal blood was collected simultaneously. Serum thyroxine (T4) and triiodithyronine (T3) and free T4 were measured in most samples. To determine whether conjugastes of T4 were presented in amniotic and allantoic fluids. T4 was measured before and after 24-hr acid hydrolysis. T4 and T3 turnover from amniotic fluid were measured using kinetic methods after intravenous or intraamniotic fluid injection of radiolabeled hormones. T3 was unmeasurable less than 15 ng/100 ml) in amniotic and allantoic fluids as well as in most fetal serum samples. T4 levels in amniotic fluids were low before 120 days of gestation (0.17 +/- 0.03 mug/100 ml; mean and SEM) concentration between 124 and 152 days was 0.33 +/- 0.03 mug/100 ml. T4 concentrations in allantoic fluid were relatively high before 120 days (0.57 +/- 0.1 mug/100 ml) and did not increase with progression of the pregnancy...


Effects of sulfadimethoxine on tissue distribution of (14C)bilirubin in the newborn and adult hyperbilirubinemic Gunn rate. Sulfadimethoxine significantly reduced plasma bilirubin levels and altered the tissue bilirubin distribution in both the newborn and the adult Gunn rat. The majority of the unbound bilirubin appeared to distribute perferentially to the intestine and liver in the newborn, whereas in the adult the unbound bilirubin was taken up primarily by the liver. The bilirubin content of brain tissue from both age groups was significantly higher after sulfadimethoxine treatment.


Exercise and asthma: overview and clinical impact. The question of the relationship between exercise and asthma dates back to the second century A.D. and was not resolved until R.S. Jones and co-authors in 1962 reported that there were in reality two effects on the asthmatic patient from exercise depending on the duration of the exercise. Short-term exercise of less than four minutes resulted in bronchodilatation, whereas longer-term exercise resulted in bronchoconstriction. Exercise-induced bronchospasm occurs in all ages but is more common in children, developing in 85% of boys and 65% of girls with severe asthma. Pulmonary function tests are valuable in identifying these patients as well as their response to various drugs. With this information specific recommendations may be made for the prevention and control of exercise-induced bronchospasm, thus allowing asthmatic children to cope with their peers in exercise programs and competitive sports.


Incidence of exercise-induced asthma in children. The incidence of exercise-induced asthma (EIA) was studied in 134 asthmatic, 102 nonasthmatic atopic, and 56 nonatopic children. Pulmonary function tests measuring forced vital capacity (FVC) and one-second forced expiratory volume (FEV1) were performed on each child prior to and serially for 20 minutes following free running exercise. The incidence of the EIA among the asthmatic and atopic nonasthmatic children was 63% and 41% respectively, and 7% among control subjects. Airway function was studied prior to and after a standardized free running exercise test. Forty-one percent of the nonasthmatic and 63% ofthe asthmatic atopic children had a significant decrease in airway function as compared to 5% of the nonallergic subjects.


Exercise-induced bronchial lability in wheezy children and their families. Studies have been caried out to investigate bronchial lability as assessed by means of standardized running exercise tests. It was found that children who had been wheezy as infants had an abnormal degree of bronchial lability, even if they were asymptomatic, and closely resembled children who had continued to wheeze. The relatives of asthmatic children displayed a high incidence of bronchial lability, atopic diseases, and positive skin tests, and a similar incidence was found in the relatives of babies with wheezy bronchitis. The difference from a control population was particularly noticeable in the healthy relatives of wheezy children. Studies in monozygotic twins have shown a very high incidence of concordance for asthma, particularly when bronchial lability is taken into account. These studies suggest a common genetic basis for wheezing in childhood, based on the inheritance of bronchial lability. The difference in responses of the actively asthmatic subject from that of other labile subjects suggests that additional environmental factors are necessary to interact with the inherited bronchial lability.


Exercise-induced asthma in children with intrinsic and extrinsic asthma. The significance of intrinsic asthma as a diagnosis has been questioned since, except for the lack of evidence of allergy, there is no pathophysiologic distinction from extrinsic asthma. Following a standardized exercise stress, allergic (extrinsic) asthmatics respond with more severe asthma than do nonallergic (intrinsic) asthmatics. The incidence of significant exercise-induced asthma is greater in a group of extrinsic asthmatics than in intrinsic asthmatics. Extrinsic asthmatics show increased airway sensitivity during a significant pollen season.


Relative sensitivity of different pulmonary function tests in the evaluation of exercise-induced asthma. Sixty asthmatic children were exercised on a bicycle ergomete rand had pulmonary function tests perormed before and repeatedly after exercise. Pulmonary function measurements included airway resistance (Raw), specific airway conductance (SGaw), functional residual capacity (FRC), peak expiratory flow rate (PEFR), maximum mid-expiratory flow (MMEF), forced expiratory volume during first second of expiration (FEV1), and forced vital capacity (FVC). At any one time during the post-exercise observation period decreases in SGaw were greater than changes in any other pulmonary function test, making SGaw the most sensitive test for the detection of exercise-induced airway obstruction in asthmatics. Beyond five minutes after exercise PEFR and MMEF were reduced by exercise approximately equally, but somewhat less often and less markedly than SGaw. Exercise-induced reductions in FEV1 were less marked and less frequent than decreases in PEFR and MMEF, and reductions in FVC were the least severe and least often observed abnormality. Decreases in SGaw were significantly, but not linearly correlated with decreases in PEFR, MMEF, FEV1, FVC, and FEV1/FVC. There were statistically significant linear correlations between exercise-induced increases in FRC and decreases in FVC and between increases in Raw and FRC. If we accept that increases in Raw and FRC indicate increases in large and small airway obstruction respectively, exercise-induced decreases in FVC may indirectly suggest acute hyperinflation and thus small airway obstruction. Although the positive correlation between Raw and FRC indicated that both large and small airway obstruction developed after exercise in many of our asthmatics, increases in Rae were usually greater than increases in FRC, suggesting that large airway obstruction tends to be greater than small airway obstruction in exercise-induced asthma.


Grading, time course, and incidence of exercise-induced airway obstruction and hyperinflation in asthmatic children. We evaluated clinical status and pulmonary function in 60 perenially asthmatic and 11 normal children before and repeatedly after incrementally increasing bicycle ergometer exercise. The changes in clinical status and pulmonary function which were elicited by strenuous exercise were graded by an air exchange and a physiological grading system respectively. Normal children showed no significant clinical or physiological changes after exercise. Strenuous exercise elicited significant deteriorations in clinical and physiological measurements in 36% to 77% of asthmatic girls and 46% to 90% of asthmatic boys, the frequency depending on the test used to determine exercise-induced abnormalities. The incidence of exercise-induced asthma (EIA) was statistically significantly higher in asthmatic boys than girls. The higher incidence of EIA in boys was primarily due to a larger number of very severe attacks in boys than girls; mild and moderate EIA was about equally common in the two sexes. Most patients with EIA developed large and small airway obstruction, although large airway obstruction tended to be the predominant and the more severe abnormality. Clinical and physiological abnormalities, regardless of severity, were usually most marked during the first ten minutes after exercise and lessened thereafter. Mild EIA usually lasted for only 15 minutes or less; severe EIA improved, but usually did not resolve within 35 minutes of exercise. There were three patinets in whom the severity of EIA got worse after exercise and an additional seven in whom the improvement was minimal. In these ten patients isoproterenol aerosol terminated EIA, indicating that exercise-induced large and small airway obstruction in asthmatic children is primarily, if not solely, due to bronchospasm.


Blood gas in exercise-induced bronchospasm: a review. Exercise-induced bronchospasm (EIB) in some cases of asthma is related to hypocapnia, hypoxemia, and acidosis, but studies have shown that children do not develop as abnormal PCO2, PO2, or pH levels with the induction of EIB. Gradient changes of alveolar-arterial oxygen differences reveal ventilation perfusion abnormalities existing at the onset of exercise in those patients who do develop EIB.


A comparison of pulmonary function tests in detecting exercise-induced bronchoconstriction. In an attempt to compare most of the available pulmonary function tests in detecting airway obstruction after exercise, two studies were conducted. In the first study 24 bronchodilator-dependent asthmatic boys were evaluated before treadmill exercise (baseline) and at 7 and 30 minutes afterwards. The following pulmonary function parameters were measured: forced vital capacity (FVC), forced expiratory volume in one second (FEV1), maximum mid expiratory flow (MMEF), peak expiratory flow rate, thoracic gas volume, airway resistance, specific airway conductance (SGaw), and closing volume (CV). Results showed that SGaw, MMEF, and CV were the most sensitive parameters reflecting changes in airway caliber. Less significant changes also appeared in FEV1 and in FVC. The significance of these changes and their relationships to other parameters are discussed. In an attempt to better understand the effects of airway obstruction on the maximum-expiratory flow-volume curve (MMEF curve) after exersise, a second study was conducted. Comparisons were made between "classical" parameters such as MMEF (measured by spirometry), SGaw (measured by body plethysmography), and flow-volume parameters (measured by wedge spirometer). Sixteen asthmatic subjects (9 to 12 years of age) whose airway obstruction was further exacerbated by exercise of a moderate work load on the treadmill (2 w/kg of body weight) were studied. The asthmatic subjects were tested prior to exercise and at 7 and 30 minutes after exercise. All the measurements mentioned above were done in a randomized manner. When both MMEF and SGaw were decreased in the post-exercise period, significant correlations were obtained between these "classical" parameters and all of the flow-volume parameters. However, when only one of the two was decreased, no correlation could be obtained with any of the flow-volume parameters. It was concluded that under certain conditions flow-volume curves reflect airway obstruction satisfactorily, but under other conditions they appear to be less sensitive than the "classic" parameters. These results are also discussed.


Free running test for exercise-induced bronchospasm. The free running test has been successfully carried out in patients from 5 to 21 years old. It has the following advantages: (1) it simulates normal exercise pattern; (2) it is readily performed in any health care setting; (3) it is simple to perform and noninvasive in nature; and (4) it reveals a maximum decrease in forced expiratory volume at one second at five minutes after the exercise period. There seems to be a seasonal variation that may alter outcome of tests.


The use of the treadmill for assessing exercise-induced asthma and the effect of varying the severity and duration of exercise. Running produces a greater amount of post-exercise bronchoconstriction than other forms of exercise carried out a similar metabolic rates. The treadmill can be used to provide a standardized form of exercise when studying the asthmatic child. The severity of post-exercise bronchoconstriction depends upon the rate of working on the treadmill and the duration of exercise. A maximum response is obtained by six minutes of running at an uphill slope of 10% at a speed of 5 kmph (3 mph). Even with this type of test, there is still considerable variation in the response from time to time and greatest reporducibility is obtained by repeating the test within one week. Treadmill exercise tests may be used in the diagnosis of asthma and in the assessment of its likely severity.


The cycloergometer as a system for studying exercise-induced asthma. Although bicycle exercise induces less asthma than does treadmill running, the cycloergometer offers definite advantages for quantitative testing of bronchospastic response, extensive knowldge of normal responses, lack of training artifact, and ease of physiologic monitoring. Using maximal heart rate to tailor the exercise load to a given subject, reliable responses can be obtained from subjects as young as 7 years old. To obtain such results, diurnal variations in response to exercise, resting bronchial tone, and previous medication must be controlled, and pulmonary function measurements must be taken frequently after exercise.


Comparative aspects of available exercise systems. Comparative studies have demonstrated that running is the greatest stimulus to exercise-induced asthma (EIA) and is the preferred laboratory test model to examine EIA and its modifications. Swimming provokes least EIA and is the exercise of choice for asthmatics. The frequency and magnitude of EIA produced by running is not altered by regular swimming training.


The correlation between exercise-induced bronchoconstriction and bronchial methacholine sensitivity in asthma. A possible relationship between exercise-induced bronchoconstriction (EIB) and bronchial sensitivity to the cholinergic drug, metacholine, was studied. On the same day, 57 asthmatic subjects inhaled increasing concentrations of the drug and were then tested for EIB by exercising to exhaustion. A fall in peak expiratory flow rate of 15% or more was considered a positive reaction to either test. Of the 45 patients exhibiting a positive methacholine reaction, 33 showed bronchoconstriction after exercise (including three of the ten who had no previous history of exertional asthma) while none of the 12 methacholine nonreactors developed EIB. This significant correlation (P is less than .001) between the tendency to develop EIB and sensitivity to methacholine suggests that bronchoconstriction, induced either by exercise or methacholine, is a specific reaction.


New drug evaluation using exercise-induced bronchospasm. Exercise-induced asthma was used to demonstrate that a xanthone, a new orally active cromolyn-like drug, has significant activity in man. The effect of a single oral dose of 8 mg/kg was studied in a double-blind corss-over fashion at two and five hours after administration, with significant inhibition of post-exercise bronchospasm being seen at five hours. Further study seems indicated to determine optimal dosage and clinical effectiveness.


The effect of pretreatment with atropine in exercise-induced bronchoconstriction. Because asthmatics who exhibit exercise-induced bronchonconstriction are also known to be sensitive to the cholinergic drug methacholine, an attempt was made in this study to block exercise-induced bronchoconstriction in nine asthmatics with a known history of exercise-induced bronchoconstriction by pretreatment with atropine. Inhalation of atropine before exercise produced milder exercise-induced bronchoconstriction in three of the nine asthmatics and a total inhibition of exercise-induced bronchoconstriction in two patients. The remaining four asthmatics still exhibited bronchoconstriction during exercise, despite pretreatment with atropine. There was no correlation between a past history of reagenic bronchial allergy or aspirin hypersensitivity and the extent of atropine inhibition of exercise-induced bronchoconstriction. This varied effect of atropine was considered to be due in part to varying amounts of the drug reaching those parts of the bronchial tres most involved in triggering exercise-induced bronchoconstriction.


Airway smooth muscle in exercise-induced bronchospasm: some speculations. Some possible neurophysiological, biochemical, and pharmacological pathways affecting the state of contractility if airway smooth muscle in exercise-induced bronchospasm (EIB) are described. No unifying hypothesis can be set forth at this time. Indeed, it is likely that the heterogeneous nature of EIB is a reflection of the numerous biochemical loci in smooth muscle cells that could be affected by the various metabolic changes accompanying heavy exertion.


Visual decentration: from stereometric points to planeometric forms. 19 3-yr.-olds chose to put blocks together to produce good continuation across stereometric edges. 4 mo. later they began to produce good continuation across planeometric edges. A group of 15 4-yr.-olds chose to produce symmetrical forms rather than simply to align edges. Symmetrical form was produced across planeometric surfaces but not across stereometric contours. These data suggest that visual decentration is first a shift from looking at single points touched to single points displaced from touch and, at a still later age, a shift to the organization of many points. The failure of the older group to produce a symmetrical form across stereometric contours could have resulted from the cultural press to attend to planeometric information.


Effect of threat of shock for being disfluent of fluency of normal speakers. Each of 20 adult nonstutterers read a 330-word passage six times with a one minute pause between readings. Between the third and fourth reading or the fifth and sixth reading (determined by a table of random numbers) Ss were informed that at the conclusion of the next reading an electric shock would be administered for each instance of disfluency detected by E during that reading. Ss did not become less fluent as a consequence of the experimental condition. Their mean disfluency frequency during the threat-of-shock reading was lower than during the preceding one. Thus, threat of shock for being disfluent may be a condition which differentiates stutterers from nonstutterers since stutterers have been reported to become less fluent under this condition.


Note on conservation differences for Indian and white children. Piagetian tasks for the conservation of length and volume were administered to two groups of 7-and 8-yr.-old Canadian Indian and white children (ns = 35, 33). There was no significant difference across groups for the conservation of length; however, conservation of volume and the ability to conserve both length and volume resulted in a higher proportion of successful performances for the white group.


Aesthetic complexity and exploratory behavior. To test the hypothesized relationship between exploratory behavior and stimulus complexity, 15 paintings comprising three levels of complexity were derived from ratings by 30 Ss. A further 30 Ss were requested to view the compositions for as long as they wished, and a third group of 30 Ss were asked to write any questions they might have in response to each painting. Data showed a monotonic relationship between complexity and looking time. Total questions and specific interrogations were most frequent for complex stimuli. Yes-no questions did not vary with rated complexity.


Perceptions and behaviors of dogmatic subjects in a T-group setting. Ss scoring 1 SD above or below the mean of the Rokeach Dogmatism Scale, Form E participated. During 12 hr. of training, Ss' behaviors were recorded by observers and each S was periodically rated by his peers on his perceived level of contributions to the group process. Significant differences were found between high and low dogmatics in the behaviors exhibited during the T-groups. Low dogmatics were more self-revealing, more attentive to the here-and-now aspects of the T-group, less apt to give negative feedback, and less likely to reject the group. A significant curvilinear relation was found between peer ratings and behavior in the T-group, with Ss who were rated extremely by their peers being more self-revealing, more attentive to the here-and-now of the group, more apt to give negative feedback, and less likely to reject the group verbally.


Effects of task difficulty on subsequent preference for visual complexity. Ss rated on a 1 to 13 Likert scale their preference for four environmental scenes which differed in complexity. Ss were then given 30 sec. to solve each of 10 3-, 5-, or 7-letter single-solution anagrams. Finally the Ss rated four new slides which were of the same complexity level as the slides seen during the first session. Change scores were calculated by subtracting the preference ratings at each complexity level during the first session from the rating at the corresponding complexity level during the second session. The Anagram Complexity X Slide Complexity interaction was significant: the complexity of the slides showing the most positive change scores was inversely related to the complexity of the anagrams attempted. The results were discussed in terms of optimal level of stimulation theories.


Further evaluation of a sensitive method for detecting experimental brain lesions. Small, unilateral cortical lesions were placed in either medial-anterior, lateral-anterior, or posterior cortex of 20 male, Sprague-Dawley, albino rats. In addition, unilateral sham operations were performed on 8 rats from the same stock. All subjects had been trained prior to surgery with diametrically opposed visual habits mediated by opposite cerebral hemispheres. The opposing habits were, insofar as possible, evenly matched prior to surgery. After surgery, lesioned hemispheres, regardless of locus of lesion, were slower in acquiring the habit they mediated, than were unlesioned hemispheres. Further, unlesioned hemispheres dominated in test trials in which subjects chose one of the two cue doors with both eyes open. These findings confirm that the "interocularly conflicting discrimination" baseline detects a role of extrastriate cortex in mediation of pattern discrimination habits. They further indicated that losses occur without regard to the various locations of lesion employed.


Determinants of visual attention in real-wound scenes. The present study examined the saliency of size, movement, and human content variables in visual selective attention. Ss named stimuli present in motion pictures of real world scenes or in animated cartoon controls during a 15-sec. exposure period. Regardless of the type of presentation that they saw, Ss tended to name large and/or moving stimuli more often than small and/or nonmoving stimuli. Also, small human stimuli were named more frequently than small nonhuman stimuli, while there were no differences between the frequencies with which large human and nonhuman stimuli were named. The order in which Ss named stimuli was not related to either the size, movement, or human content variables. Results are discussed in terms of the generalizability of the results of previous studies to conditions simulating the real world.


Cannabis intoxication: effects of monetary incentive on performance, a controlled investigation of behavioural tolerance in moderate users of cannabis. The study examined the effect of monetary incentive on the performance of five tasks after two heterogeneous groups of experienced users of cannabis and previously naive subjects had smoked placebo material and two dose levels of cannabis. The performance of the motivated subjects was compared with that of two non-motivated matched groups. Dose-related impairment was found on four of the five tasks, supporting previous findings of cannabis-induced impairment of short-term memory, goal-directed behavior and choice reaction times. Results for three of the tasks suggested that the performance of the motivated subjects was less affected by the drug than was the performance of the non-motivated subjects. There was no difference between the performance of subjects naive and experienced with respect to drug use.


Sex, sex-role identity, and cognitive style. Two studies were conducted to test competing theoretical predictions concerning the relationships among sex, sex-role identity, and field independence: (1) Witkin and Vaught's positions that maleness and masculinity (regardless of biological sex) are associated with field independence and (2) the present author's contention sex reversed sex-role identity, irrespective of sex, would be predictive of field independence. Data collected from 110 high school males and 78 college females and males supported the latter prediction but only for males. No main effects due to sex were found.


Relationship between age, socioeconomic status, and field independence. The hypothesis was supported that socioeconomic variables confound the relationship between age (20 and 60 yr.) and field independence measured on the rod-and frame task for 54 adult women (nurses).


Personality, reinforcement and learning. Conflict in predictions resulting from Eysenck's (1957) and Gray's (1970) theoretical formulations on personality and conditioning were tested at the behavioral level. Given conditions which do not produce over-arousal, it would be predicted from Eysenck's position that Introverts would condition better than Extraverts. From Gray's formulation it would follow that Introverts condition better if negative reinforcement is used and Extraverts condition better if positive reinforcement is used. The two opposing predictions were tested in pursuit rotor learning by either positively or negatively reinforcing the hit/miss dimension of performance by 166 males aged 14 to 15 yr. The results gave support to Gray's position but if over-arousal is assumed Eysenck's position is tenable.


Effects of hypoxia on peripheral visual response to dim stimuli. Response times (RTs) of 9 Ss were obtained for detection of 48 flash stimuli distributed throughout the visual field during 3 1/4-hr. exposures to each of 4 hypoxia conditions (0, 13,000, 15,000, 17,000 feet equivalent elevation). The luminances of all stimuli were set in common at the detection threshold value for the visual periphery. RTs were impaired in direct relation to hypoxic exposure severity, the peak impairments occurring within 90 min. followed by gradual recovery. Since the present results showed less impairment than previous data for brighter stimuli using the same task, it is concluded that stimulus contrast is more critical to peripheral signal detection than absolute stimulus luminance, particularly under hypoxic exposure.


Differences in creativity among homogeneous groups of students using the Torrance Test of creative Thinking and the Welsh Figure Preference Test. Three intact groups of students in Grade 5 (n = 79) were grouped by ability on the Metropolitan Achievement Test and given the Torrance and Welsh tests used to assess creativity. Middle ability children scored higher on fluency, flexibility, and origence than the other two groups.


Perception of curves.

Perception of curves. In 4 experiments, in which 66 subjects participated, the perception of 6 types of curved contours in two-dimensional shapes was studied. Random polygons and their curvilinear transformations were presented for detection under low-luminance contrast conditions, oddity-type discrimination problem solving, tachistoscopic identification, and identification involving visual acuity in distance vision. In all experiments curvature affected perception at statistically significant levels, but the extent of this effect was a function of (1) the locus and direction of curvature, (2) the level of compactness-jaggedness of the figure, and (3) the nature of perceptual task. Shapes with acute corners were more easily perceived than shapes with curved corners. Within these two classes of shapes, those with convex sides were perceived as having greater curvedness than those with concave contours. However, the degree to which curvature affected response was determined primarily by the nature of the perceptual task.


Blinking and thinking.

Blinking and thinking. Blinking is related to certain cognitive processes. For example, individuals "punctuate" their speech by blinking between phrases and at the end of sentences. Daydreaming is associated with low rates of blinking. Blinking occurs between fixations and may be timed so as not to interfere with significant visual input. Apparently, blinking occurs at transitions between internal events and is inhibited at other times. In the experiment reported here, blinking was measured while the activity of operational memory was manipulated with mental load kept constant. The rate of blinking was significantly reduced when the cognitive operation of internal counting was being performed. It is inferred that the blink rate is low when information in memory is being operated on. To suspend blinking during certain cognitive activities would be adaptive if blinking disrupts them. Since the blackout period of the blink produces a rapid change in visual level, blinking disrupts those cognitive processes utilizing display areas accessible to visual input. Operational memory and the visual imagination may share components with the visual perceptual system. To protect these vulnerable processes from interference, blinking may be inhibited when they are active.


Use of a single gibbon in laboratory research. The high cost of behavioral research with primates may be partially reduced by borrowing animals from a zoo. This report describes such an arrangement involving an adult female gibbon. Operant conditioning data are presented to show that an isolated animal may be used for research.


Duration of experienced expansion and contraction of a circle. The stimulus was a white outline circle which for 60 msec. was projected onto a screen 2 m. from S. The diameter of the circle was 10 cm. and the circle line was approximately 1.5 mm. wide. Fixating the center of the circle Ss reported experiencing the circle as expanding from the point of fixation to full size and then contracting to the point of fixation. Ss' experiences fell in two classes. The durations of Ss' experience of expansion-contraction were measured in msec. Ss also participated in experiments in which they fixated the nethermost point of a 17-cm. vertical straight line which was 2 mm. wide. Ss experienced the line as lengthening from the point of fixation to full length and then shortening to the point of fixation. For a given S the duration of the experienced expansion-contraction of the circle and the experienced lengthening shortening of the straight line were the same.


Underestimation of length by subjects in motion. To check a prior observation, in the present experiment, subjects made estimates of the lengths of both the guidelines and the spaces between guidelines on automotive highways so the magnitude of the illusion could be more accurately determined. Ten males and ten females were individually tested at 0 and 60 mph. At 60 mph, spaces were estimated with an error of 85%; lines were estimated with an error of 72%. Combining data for both stimuli, an error of 78% results, which corresponds to underestimation by a factor of 4.67. This illusory effect is considerably greater than that of the moon illusion, considered by many the most powerful of the classical illusions.


Observing behavior in the albino rat: a within-subjects' comparison of increasing levels of visual complexity. 54 male CD strain Charles River albino rats were tested for complexity preference using a non-locomotor response. Each subject was presented an array of photographic slides containing an incremental series of complexity elements, i.e., 1, 2, 5 and 25 elements. All photographic slides were randomized both within and across subjects. Each photographic slide of each level of complexity was repeated three times. Results of an analysis of covariance, with luminance as the covariate, showed that cummulative viewing time increased with an increase in stimulus complexity. The relationship between looking and level of complexity was shown to be different when covariance procedures were used than without them.


Relationships among perceptual-motor, self-concept, and academic measures for children in kindergarten, grades one and two. Most studies reporting correlations of psychomotor and cognitive measures deal with perceptual-training programs for the remediation of academic deficiencies. This study provided additional information about the relationships of perceptual-motor ability and academic achievement for children in kindergarten through the second grade, and included self-concept. A small positive relationship between perceptual-motor ability and academic ability was observed for all three grade levels. There was little indication of any significant relationship between self-concept and academic ability, which provides no basis for the hypothesis proposing self-concept as an intervening variable in the relationship between the perceptual-motor and academic areas, at least for such young subjects.


Developmental study of human visual and auditory evoked responses. The present study investigated the development of visual evoked response by varying the physical factor of photic stimulus size and the psychological factor of age and the development of auditory evoked response was investigated on habituation trials, using the subjects of wide range in ages. The results showed that visual evoked response decreased in amplitude as a function of age and increased as a function of stimulus size and the auditory evoked response also decreased in amplitude as a function of age, while no prominent habituation of evoked response over the trial blocks was observed.


Personality correlates of the Stroop Color and Word Test: more negative results. Numerous studies have investigated personality correlates of performance on the Stroop Color and Word Test. With one exception, these studies have failed to identify any significant correlates between the two variables. The present study examined this relationship by administering the Stroop Color and Word Test, Cattell's 16 PF, and the Maudsley Personality Inventory to a sample of 210 college students. Results indicated no significant correlates existed between five different measures of Stroop Color and Word Test performance and the personality scales. It was concluded that the Stroop Color and Word Test has little relationship to measures of personality and may best be understood in terms of specific cognitive processes.


Measures of visual-auditory integration and their relations to reading achievement in early grades. Several tests of visual-auditory integration were designed to represent the various tasks that have been found to correlate with reading achievement in the early grades. The battery of tests was administered to about 100 first, 84 second, and 110 third graders. Several questions were posed: (1) Do the various tests of integration measure the same underlying ability or are there separate abilities that are tapped by the various tests? (2) Are the various tests correlated with reading because they tap a common integrative ability that is related to reading or are there several abilities, each related to reading independently to the others? (3) To what extent are the relationships between the integrative abilities and reading separate from the relationship between reading and IQ? Results indicated that there are separate integrative abilities, and multiple regression analyses showed that several of the integrative measures were correlated with reading independently of the other measures. Further, several of the integrative measures accounted for significant reading variance beyond that accounted for by IQ tests. The results were discussed in the context of the need to differentiate adequately between various patterns of perceptual weakness in the diagnosis and remediation of perceptually based reading difficulties.


Differential reinforcement of reaction times in developmental retardates. The effects of differential reinforcement on reaction times to two intensities of reaction signal were investigated in 36 developmental retardates. One group was reinforced for reaction times to the higher intensity signal; a second group was reinforced for reaction times to the lower intensity signal; a third group received no reinforcement. Reaction time decreased as signal intensity increased and decreased over sessions as a function of reinforcement. Likewise, reinforcement either exaggerated or reversed differences in reaction times to each signal depending on the particular reinforcement contingency. Results were discussed in terms of attentional and motivational explanations of effects of signal intensity on reaction times of retardates.


Inhibitory effect of a second stimulus on reaction time to a primary stimulus: a partial successful replication. A second visual stimulus, to which no response was required, following a primary one lengthened the reaction time to the first stimulus for 25 college students. Reaction time rose as a function of the increase in the interstimulus interval. Duration time of the second stimulus did not affect this response. These results were found under a condition of stimulus-response certainty.


Perception of less irregular and more irregular stimuli. Berlyne's stimuli were presented in a stereoscope to test his hypothesis that Ss prefer the more irregular member of a pair of stimuli. 32 Ss, screened for normal acuity and phorias, were tested. Contrary to Berlyne's hypothesis and consistent with earlier stereoscopic studies, Ss tend to report more frequently the less irregular member of a pair of stimuli. These data can be explained by an information-conflict model; however, the clearest implication of this study was that experiments concerned with familiarity of stimuli should be qualified by the method.


Use of classical conditioning procedures to control aggressive behaviors in children: a preliminary report. This experiment tested the effectiveness of classical conditioning procedures in reducing children's aggressive behaviors in a classroom setting. As hypothesized, 11 boys and 14 girls who were shown pictures of aggressive scenes paired with the presentation of negatively evaluated words subsequently displayed significantly fewer aggressive responses than controls who had not experienced these conditioning procedures. A significant teacher/classroom effect was also found, which appeared to be a function of the level of the 3 teachers' permissiveness. Specifically, children's aggressive behaviors occurred more frequently in the classroom where a permissive atmosphere prevailed than in the classroom where a more restrictive atmosphere was maintained. In those classrooms where a permissive atmosphere prevailed, the children who were the same sex as their teacher tended to display more aggressive responses than those children who were the opposite sex from their teacher.


Perceptual stability, image size, binocularity and creativity. 24 male and female Ss, divided into three groups of 8 Ss each on the basis of their creativity test scores, were tested under fully counterbalanced conditions involving three sizes of Necker cubes, viewed under both monocular and binocular conditions. The response measure was number of figure of reversals experienced during 2-min. viewing periods for each set of conditions. Image size was statistically significant, while creativity levels and viewing conditions were not.


Humor response as related to violation of expectancies and to stimulus intensity in a weight-judgment task. In a study of humor disguised as a psychophysical task, 60 subjects judged an initial series of 10 weights which was either light or heavy and then a shift weight which was either light, medium, or heavy. Degree of humorous response varied with the discrepancy of the shift weight and its absolute magnitude.


Porteus Maze Q score as a measure of impulsivity. The validity of the Q score (derived from the qualitative scoring of the Porteus Maze Test) as a measure of impulsivity was investigated by comparing the performance of high- and low-impulse groups. 80 Ss were employed and the Q score failed to differentiate high- from low-impulse institutionalized Ss, delinquent from nondelinquent Ss and extreme groups based on ratings. These findings are at variance with the claim that the Q score measures impulsivity.


The methylation state of poly A-containing messenger RNA from cultured hamster cells. The poly A-containing mRNA of cultured hamster (BHK-21) cells has been examined with regard to methylation status. Steady state-labeled mRNA was obtained by incubating cells for 20-22h in the presence of [methyl-3H]-methionine and 32Pi. The degree of methylation of this RNA was 1.8 methyl groups per 1000 nucleotides, or 4-5 methyl groups on the average per molecule. The nature of the methylated residues was determined by paper chromatography and electrophoresis of acid and alkaline hydrolysates, by DEAE cellulose chromatography of alkaline hydrolysates and of T2 RNase digests, and by examining the effect of subjecting samples to "beta-elimination." Approx. half of the methyl groups occurred in standard ("internal") linkage, 10% as m5Cp and 40% as m6Ap residues. The remainder occurred at least for the most part in "blocked" 5'-termini with the presumptive structure m7G(5')ppp(Nm)p.., where Nm was Gm, m6Am, Um, or Cm.


DNA methylase from HeLa cell nuclei.

DNA methylase from HeLa cell nuclei. A DNA methylase has been purified 270-fold from HeLa cell nuclei by chromatography on DEAE-cellulose, phosphocellulose, and hydroxyapatite. The enzyme transfers methyl groups from S-adenosyl-L-methionine to cytosine residues in DNA. The sole product of the reaction has been identified as 5-methylcytosine. The enzyme is able to methylate homologous (HeLa) DNA, although to a lesser extent than heterologous DNA. This may be due to incomplete methylation of HeLa DNA synthesized in vivo. The HeLa enzyme can methylate single-stranded DNA, and does so to an extent three times greater than that of the corresponding double-stranded DNA. In single-stranded M. luteus DNA, at least 2.4% of the cytosine residues can be methylated in vitro by the enzyme. The enzyme also can methylate poly (dG-dC-dG-dC) and poly (dG, dC). Bilateral nearest neighbors to the 5-methylcytosine have been determined with M. luteus DNA in vitro and HeLa DNA in vivo. The 5' neighbor can be either G or C while the 3' neighbor is always G and this sequence is, thus, p(G/C)pmCpG.


Visualization of planar drug intercalations in B-DNA. A computerized linked-atom modeling system was developed to examine the stereochemical requirements for intercalation of planar drugs into DNA. All classes of conformational possibilities for extending the polynucleotide backbone were examined for their ability to accommodate insertion of a drug into a base-paired region of DNA compatible with adjacent regions of B-DNA while stacking interactions, steric strain and non-bonded interatomic contacts were optimised. One conformation was found which proved superior to all others in ability to satisfy these criteria: an extension of the backbone by characteristic changes in two torsion angles to trans values, plus a change in one sugar puckering to C3'-endo to relieve strain in an adjacent residue. The turn angle distributed over three polynucleotides for this most general mode of intercalation is 90 degrees, equivalent to a helical unwinding of -18 degrees for B-DNA.


Synthesis of tobacco mosaic virus coat protein following migration of viral RNA into isolated mouse liver mitochondria. RNA of Tobacco Mosaic Virus is shown to be able to migrate into isolated mouse liver mitochondria, whence it can be reisolated intact. The migration of RNA is accompanied by enhanced rate of protein synthesis, which is sensitive to chloramphenicol, but not to cycloheximide. Evidence is presented showing that, among the products formed is the coat protein of Tobacco Mosaic Virus.


Application of base-catalysed reaction to the synthesis of dinucleotides containing the four common deoxyribonucleosides and of oligodeoxythymidylates. The phosphorylation of nucleosides by a nucleoside phosphorofluoridate in the presence of potassium tert-butoxide is a very effective reaction for internucleotide bond synthesis in the case of pyrimidine deoxynucleosides. However, for the purine deoxynucleosides, yields are reduced due to competing reactions. The method was applied to the stepwise synthesis of oligothymidylates. The yield of the trinucleotide was good whereas that of the tetranucleotide was reduced due to the insolubility of the intermediate trinucleotide in the presence of potassium tert-butoxide.


Reaction of a nucleoside 2, 4-dinitrophenyl phosphate with fluoride; a convenient method for the preparation of the nucleoside phosphorfluoridate. Examination of the reaction of 2, 4-dinitrofluorobenzene with thymidine-5' phosphate in detail reveals that the initial product is the 2, 4-dinitrophenyl ester. This reacts with fluoride to produce thymidine-5' phosphorfluoridate. This second reaction provides the basis for the conversion of preformed thymidine-5' 2, 4-dinitrophenyl phosphate to thymidine-5' phosphorofluoridate.


An approach to histone nearest neighbours in extended chromatin. The primary sequence organization of histones upon the DNA molecule in chromatin has been analyzed by extension of the nucleoprotein at very low ionic strength and crosslinking with a reversible crosslinking reagent, methyl-4-mercaptobutyrimidate. Histones extracted after limited reaction were fractionated into different classes and the composition of the oligomers analyzed after reduction of the crosslinked material. We have found that the following dimers occur at a high frequency: (F3-F2b), (F3-F2a2), and (F2b-F2a2), whereas (F2b-F2al), (F3-F2al) and (F3-F3) occur with a lower frequency. F1 appears to polymerize rapidly to largely homogeneous polymers of high molecular weight. These results are analyzed in terms of several models proposed for chromatin structure.


A facile method for the preparation of N6-substituted ATP-Sepharose. The attachment of 6-methylsulfonylpurineribose 5'-triphosphate to AH-Sepharose 4B as a facile method for the preparation of ATP-Sepharose is described. This reaction is compared to the coupling of 6-chloropurineriboside 5'-triphosphate as well as N(6)-aminocaproyl-ATP. An improved synthesis of purineriboside 5'-phosphates which does not require purification by column chromatography is also described.


The estimation of affinity constants for the binding of model peptides to DNA by equilibrium dialysis. The binding of lysine model peptides of the type Lys-X-Lys, Lys-X-X-Lys and Lys-X-X-X-Lys (X = different aliphatic and aromatic amino acids) has been studied by equilibrium dialysis. It was shown that the strong electrostatic binding forces generated by protonated amino groups of lysine can be distinguished from the weak forces stemming from neutral and aromatic spacer amino acids. The overall binding strength of the lysine model peptides is modified by these weak binding forces and the apparent binding constants are influenced more by the hydrophobic character of the spacer amino acid side chains than by the chainlength of the spacers.


Replication of double-stranded RNA in particles of Penicillium stoloniferum virus S. RNA polymerase activity was assayed in different particle classes of Penicillium stoloniferum virus S. RNA polymerase activity was found to be associated with H particles, which contain double-stranded RNA and single-stranded RNA, but not with L particles, which contain only double-stranded RNA and not with M particles, which contain only single-stranded RNA. In H particles the reaction occurred with the formation of one new molecule of double-stranded RNA (or two complementary single strands of RNA) per virus particle and the production of product particles (P particles), which contained two molecules of double-stranded RNA (or its equivalent). This RNA polymerase is therefore a replicase, which catalyses the synthesis of the two complementary strands of double-stranded RNA in a single virus particle. This is the first report of this type of RNA polymerase system.


Isolation and in vitro translation of human placental lactogen messenger RNA from human term placenta. A messenger activity for HPL was identified in normal human term placentas. The mRNA was translated in rabbit reticulocyte cell-free system. The HPL synthesized was quantified by a specific immunoprecipitation and further identified by electrophoresis on sodium dodecyl sulfate polyacrylamide gel. The HPL synthesized in the reticulocyte lysate exhibited a molecular weight between 20,000 and 22,000 daltons similar to the active hormone. The messenger RNA activity for HPL corresponded to a sedimentation coefficient of 11-12 S. Furthermore the messenger activity for HPL was preferentially associated with membrane bound polyribosomes than with free polyribosomes.


Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's. The modified nucleoside, 7-(4,5-cis-dihydroxy-1-cyclopenten-3-yl-aminomethyl)-7-deazaguanosine, designated as Q, and its derivative, Q*, were found in tRNA's from various organisms, including several mammalian tissues, other animals such as starfish, lingula and hagfish, and wheat germ. Q isolated from rat liver tRNA was found to be identical with E. coli Q by mass spectrometry and thin-layer chromatography. Thus the rare modified nucleoside Q originally isolated from E. coli tRNA, is widely distributed in various organisms. Analysis of the mass spectrum of Q* suggested that it has a different side chain from Q.


Evidence of altered histone interactions, as investigated by removal of histones, in chromatin isolated from rat liver nuclei by a conventional method. It is shown that the release of the slightly lysine-rich histones f2a2 and f2b by 0.4 M ammonium sulfate from conventionally isolated chromatin is diminished in comparison to the lysed nuclei. The change in extractability is further demonstrated by the application of ethidium bromide. At a molar input ratio of 0.09 (moles ethidium bromide/moles nucleotide) and 0.4 M ammonium sulfate the slightly lysine-rich histones are released from the chromatin to 70 - 80% if the lysed nuclei are used. At 0.1 M ammonium sulfate ethidium bromide effected also a release of 50 % of histone f1. Comparable effects could not be observed with chromatin prepared in a conventional way but instead a tendency towards loss of histone f3 in the presence of ethidium bromide was observed.


The influence of a new benzonaphtyridone derivative (IFC-45) with antidepressant activity on the central nervous system. Part I. The influence on behaviour and bioelectrical activity of brain. IFC-45 (10-(3-dimethylaminopropyl)-7-chlorobenzo-[b]-[1,8] naphtyridone-5 monohydrochloride) in small doses, similarly as imipramine and amitryptyline, did not influence animal behaviour. In higher doses it stimulated excitability of animals while imipramine and amitryptyline appeared to have inhibiting action. After IFC-45, desynchronization in EEG was obtained whereas both imipramine and amitryptiline induced synchronization of EEG.


The influence of a new benzonaphtyridone derivative (IFC-45) with antidepressant activity on the central nervous system. Part II. The influence on the amphetamine and reserpine effects and other pharmacological studies. The Influence of a New Benzonaphtyridone Derivative (IFC-45) with Antidepressant Activity on the Central Nervous System. Part II: The Influence on the Amphetamine and Reserpine Effects and Other Pharmacological Studies. Pol. J. Pharmacol, Pharm. 1975, 27, 479--491. IFC-45 (chem. formula see p. I, page 471) potentiated amphetamine action in a similar manner as standard drugs, had significant but slightly less than the compared standards anti-reserpine activity. IFC-45 did not act anticonvulsivantly, did not affect physostigmine toxicity or evipan anaesthesia.


Toxicological evaluation of a new benzonaphtyridone derivative (IF C-45). DL50 for 10-gamma-dimethylaminopropyl-7-chlorobenzo(b)-(1-8)-naphtyridone-5 hydrochloride (IF C-45) determined on several species of animals at different ways of administration, had values similar to those of imipramine and amitryptyline. IF C-45 a-cumulates in 34-4% of A-DL50. The evaluation of chronic toxicity was performed on rats, giving IF C-45 daily for 3 months the doses of 30, 25 and 12-5 mg/kg. The results of hematological, biochemical and histological estimations did not show in these animals any marked toxic effects.


The influence of methionine on rats and mice behaviour. Methionine given ivc or ip to rats and ic to mice acts only slightly on the central nervous system of these animals; it has either stimulating or inhibiting action, depending on the test applied.


Inhibitory effects of beta-hydroxyethyl 2,4-dinitrophenyl disulphide on rat brain cholinesterase activity. Hydrolysis of 1-6 mM AChJ by rat brain homogenates in TRIS-HCl buffer, pH 7-8, supplemented with NaCl and MgCl2, was found to be suppressed by a half by pretreatment with 0-3 mM beta-hydroxyethyl 2,4-dinitrophenyl disulphide (HEDD), a reagent for SH groups. This effect has been shown to be due to partial inhibition of a Phg-sensitive esterase which has been characterized as AChE on the ground of experiments with MeCh and BuCh. Similar inhibition of AChJ hydrolysis was also noted on pretreatment of brain homogenates with 0-05 M N-ethylmaleinimide (NEM). The inhibition degree has turned out to depend upon either AChJ concentration (0-1--4-8 mM) and duration of contact of the tissue with HEDD before addition of the substrate. The AChE activity revealed in 0-2 mM AChJ was found to be particularly sensitive to HEDD. Sensitivity of rat brain AChE to either HEDD and NEM may indicate for occurring of SH in some one its isozyme. No less, other mechanisms of the inhibition, particularly possibility of interaction of HEDD with a disulphide group must be also considered.


The effect of some dithiocarbamic acids on dopamine-beta-hydroxylase and catecholamines level in rat's brain. In search for selective dopamine-beta-hydroxylase inhibitors, we studied the effect of dimethyl-, monocyclohexyl-, and dicyclohexyldithiocarbamic acids on the activity of this enzyme and on dopamine and noradrenaline levels in rat's brain. All the three derivatives inhibit the activity of dopamine-beta-hydroxylase in brain; in the tested doses, their effectiveness is similar. Moreover, all three compounds decrease noradrenaline level in brain. The tested derivatives differ from one another in their effect on dopamine level in brain. Dimethyl- and monocyclohexyl derivatives increase while dicyclohexyl derivative decreases the level of this amine.


Synthesis and pharmacological properties of new pyridine derivatives.2,6-bis-[diphenyl-(and dibenzyl)-hydroxymethyl]-piperidines. Syntheses of 2,6-bis-(diphenylhydroxymethyl)-, 2,6-bis-(dibenzylhydroxymethyl)- and 2,6-bis-(phenylbenzylhydroxymethyl)-pyridines were carried out. These compound were hydrogenated to the corresponding piperidine carbinols. The compounds are of low toxicity and were tested on mice for their CNS activity, using climbing test. 2,6-bis-(dibenzylhydroxymethyl)-piperidine (VI) exhibited central depressive activity.


Studies on absorption and elimination of drugs. Part IV. Computer evaluation of pharmacokinetic parameters of the two compartment open model. A simple programme was written in ALGOL 1204 for the Polish digital computer ODRA 1204. It permits the fitting of the biexponential equation C = Ae-alphat+Be-betat to the observed plasma or serum concentration data of a drug after a single iv injection.


Disc electrophoresis of turkey serum alkaline phosphatase isoenzymes using polyacrylamide gel. 1. Procedure. The isoenzymes of alkaline phosphatase in turkey serum were separated by disc electrophoresis using polyacrylamide gel as the supporting medium In this laboratory we have been unable to adapt polyacrylamide gel electrophoresis procedures for turkey serum that had been described by previous workers as successful in separating the isoenzymes of avian alkaline phosphatase. A rapid and repeatable procedure that yielded excellent resolution of alkaline phosphatase isoenzymes was devised. This procedure involved a step-by-step determination of the following: a suitable gel system, buffer system, staining procedure and sample preparation.


Disc electrophoresis of turkey serum alkaline phosphatase isoenzymes using polyacrylamide gel. 2. Turkey serum alkaline phosphatase zymograms. The isoenzymes of alkaline phosphatase (akp) in turkey serum were separated by disc electrophoresis using polyacrylamide gel as the supporting medium Test subjects consisted of 103 turkeys maintained for the "Vibrator" condition at Michigan State University; and 46 female turkey of the Nicholas Broad Breasted White egg laying strain. Thirteen distinct akp bands were isolated. The 13 bands were observed to occur in 13 different patterns, each pattern being classified as a separate zymogram. Specific types of zymograms were detected for "Vibrator" birds vs. Nicholas strain; females vs, males; high intensity egg production vs. low intensity egg production and molting vs. laying.


Light effects during incubation on lines of White Leghorns selected for fast and slow hatching. Eggs from White Leghorn lines selected for fat and slow hatching time were incubated in continuous light and in unlighted incubators. The fast hatching line averaged 48 hours shorter in incubation time than the slow hatching line. The light treated eggs averaged five hours earlier in hatching time than the unlighted treatment. These two effects on hatching time, one genetic and one environmental, were completely additive. No effect of either treatment on hatchability was observed after all hatchable eggs had hatched.


The effect of light and specific gravity on embryo weight and embryonic mortality. Eggs from Single Comb White Leghorn chickens were divided into two approximately equal groups with the range of specific gravities being present in each group. One group was incubated in a darkened Humidaire Hatchett while the other was incubated in an identical machine equipped with two 20 Watt fluorescent tubes which produced 8.3 X 10(3) ergs/cm.2-sec. at the surface of the eggs. The recommended temperature and humidity settings were used in both machines. After 7 days of incubation embryos from the lighted incubator were significantly (P less than .01) larger than those incubated in the dark. There were significantly more live embryos from low and high specific gravity eggs in the light than in the dark. There was no difference in embryonic mortality between groups for the eggs with intermediate specific gravities. Embryos from White Leghorn eggs incubated in the presence of light were significantly (P less than .01) larger on each day of incubation from day 2 through hatch. Eggs incubated in the lighted incubator hatched at 18 and 19 days and produced larger chicks than those hatched at 20 and 21 days in the dark.


Dietary and management procedures for development of consistent hemorrhagic symptoms in chicks. Chicks hatched from S.C.W.L. pullets housed in laying cages and fed a diet without vitamin K were compared with chicks hatched from pullets fed a similar ration supplemented with vitamin K3 at 750 mcg./kg. of feed. Eggs were incubated after 4, 5 and 7 months vitamin K depletion. Chicks were fed a basal chick starter with no source of vitamin K or the basal supplemented with various levels of sulfaquinoxaline (SQ) (0.0125-0.050%) or with 750 mcg./kg. of vitamin K3. Blood clotting time was determined on chicks at the beginning and at weekly intervals during each experiment. Blood clotting time was significantly increased by a SQ feeding, but no hemorrhages developed in chicks hatched from hens fed the diet containing vitamin K. Day-old chicks hatched after 4 months vitamin K depletion showed elevated blood clotting times. There was no significant change in blood clotting time during the experiment for chicks fed the basal. Blood clotting time was significantly reduced in one week for chicks supplemented with vitamin K and increased for chicks fed 0.0375 or 0.050% SQ. These levels of SQ also resulted in 25 and 47.5% skin hemorrhages. Five months vitamin K depletion of breeder hens resulted in increased incidence of skin hemorrhages, but blood clotting times were similar to those at four months. Seven months vitamin K depletion of breeder hens resulted in increased blood clotting times as compared with 4 months and 100% incidence of hemorrhages in chicks fed rations containing 0.0375 or 0.050% SQ.


The effect of dietary tryptophan and niacin levels on broiler breeder pullets. A total of 1500 pullets and 300 cockerels were obtained at one-day of age and reared to six weeks on a commercial-type diet. At six weeks of age, birds were fed one of the following: Diets 1, 2 and 3 contained 0.150 percent tryptophan and no added niacin, 28 mg. added niacin and 250 mg. added niacin per kg. of diet, respectively. Diets 4, 5 and 6 contained 0.112 percent tryptophan and no added niacin, 28 mg. added niacin and 280 mg. added niacin per kg. of diet, respectively. All diets contained 2800 kcal. of metabolizable energy per kg. of diet and were fed for 21 weeks. The 0.112 percent tryptophan diets reduced body weight and percent body protein but increased percent body fat. Niacin levels had no effect on any of the pullet production parameters measured. However, at 21 weeks of age 280 mg. of added niacin in the 0.150 percent tryptophan diets restricted body weights of the males.


Ectopic feather follicles in a chicken teratoma or malformation. At necropsy of an 8-week-old pullet a 0.75 X 5.0 cm. yellowish, moderately firm cigar-shaped object was seen in the greater abdominal air sac lying parallel to the border of the right hepatic lobe. A capsule of 5 tissue layers (from inside outward-stratified squamous epithelium fibrous tissue, longitudinal muslce, fibrous tissue and circular muscle) enclosed featherlike structures which had developed from the stratified squamous epithelial layer. This combination of tissue components constituted ectopic follicles and feathers in a malformation or teratoma.


Multiple-yolked eggs from a parthenogenetic stock of chickens. The frequency of double-yolked eggs in a parthenogenetic Dark Cornish line of chickens was increased in certain birds. A line was selected that had a higher frequency of parthenogenesis and double yolks than the High parthenogenetic group. Selection within the double-yolked line appeared to have reached its maximum. The amount of parthenogenesis was higher in the offspring groups with embryos in both single and double-yolked eggs. The number of eggs and yolks from birds that laid a large number of double-yolked eggs did not differ significantly from the number from birds that laid only a few double-yolked eggs. Many triple-yolked eggs were seen in this stock as well as one quadruple-yolked egg. High frequency, double-yolked egg layers laid the same number of eggs, therefore, multiple ovulations may be due to a hormone or enzyme effect.


The use of strain gage transducers to study gastroduodenal motility in turkeys. The purpose of this study was to determine if extraluminal strain-gauge transducers (SGT), which have not been tested in birds, could be used to accurately monitor gastroduodenal motility in turkeys. Recordings of gastroduodenal motility obtained from implanted SGT's were compared to recordings obtained simultaneously using measurements of intraluminal pressure (ILP), a method previously used in birds. Frequencies, durations and amplitudes of GI motility as determined by the ILP method were highly significantly correlated with those determined by the SGT method with one exception. The mean amplitude of the force of the contractions of the thin muscle of the muscular stomach was not significantly correlated with the ILP amplitude occurring during this contraction. This exception can be accounted for. In general, although recordings of the same GI events simulatneously obtained by the two methods were not identical, they were similar. Most importantly, similar information about the gastroduodenal contraction cycle was obtained by either monitoring method.


The activity of some peptide hydrolase enzymes in fresh and stored poultry semen from full sib groups of males and their relationship to fertility. The seminal plasma and sperm of fresh and stored poultry semen were analyzed for the presence of eight peptide hydrolase enzymes. Five enzymes: carboxypeptidase A, carboxypeptidase B, chymotrypsin, glycylglycylglycine hydrolase and pepsin were not present in either plasma or sperm. An aminopeptidase-like and a cathepsin-like activity were found in seminal plasma and sperm while a trypsin-like activity was found in sperm only. There was a significant difference between full sib groups with respect to aminopeptidase-like activity in fresh and stored plasma, while storage for 24 hours resulted in a significant increase in trypsin-like activity of sperm. The aminopeptidase-like activity of fresh sperm was positively correlated with duration and percent fertility of fresh semen, while neither cathepsin-like activity nor trypsin-like activity were correlated with fertility of fresh or stored semen except for a positive correlation between the cathepsin-like activity of fresh plasma and percent fertility of fresh semen.


Proportions of ammonia, urea, urate and total nitrogen in avian urine and quantitative methods for their analysis on a single urine sample. We have developed a dilution method for avian urine and modified existing analytical methods for ammonia, urea, uric acid and total nitrogen to permit determination of all these compounds on aliquots of the same urine sample. The proportions of urinary nitrogen in these compounds were: uric acid 55-72%, ammonia 11-21% and urea 2-11%. This ranking of proportions was consistent for four different dietary protein-water availability regimes. There were no significant differences in the proportions of these nitrogenous compounds due to either dietary protein intake or changes in water availability.